Dramatic Increase in Expression of a Transgene by Insertion of Promoters Downstream of the Cargo Gene

For expression of genes in mammalian cells, various vectors have been developed using promoters including CMV, EF-1α, and CAG promoters and have been widely used. However, such expression vectors sometimes fail to attain sufficient expression levels depending on the nature of cargo genes and/or on h...

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Bibliografiske detaljer
Main Authors: Sakaguchi, Masakiyo, Watanabe, Masami, Kinoshita, Rie, Kaku, Haruki, Ueki, Hideo, Futami, Junichiro, Murata, Hitoshi, Inoue, Yusuke, Li, Shun-Ai, Huang, Peng, Putranto, Endy Widya, Ruma, I. Made Winarsa, Nasu, Yasutomo, Kumon, Hiromi, Huh, Nam-ho
Format: Artigo
Sprog:Inglês
Udgivet: Springer US 2014
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4067539/
https://ncbi.nlm.nih.gov/pubmed/24526517
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12033-014-9738-0
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