Live-cell imaging of Pol II promoter activity to monitor gene expression with RNA IMAGEtag reporters

We describe a ribonucleic acid (RNA) reporter system for live-cell imaging of gene expression to detect changes in polymerase II activity on individual promoters in individual cells. The reporters use strings of RNA aptamers that constitute IMAGEtags (Intracellular MultiAptamer GEnetic tags) that ca...

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Detalhes bibliográficos
Main Authors: Shin, Ilchung, Ray, Judhajeet, Gupta, Vinayak, Ilgu, Muslum, Beasley, Jonathan, Bendickson, Lee, Mehanovic, Samir, Kraus, George A., Nilsen-Hamilton, Marit
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2014
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4066748/
https://ncbi.nlm.nih.gov/pubmed/24753407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku297
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