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Probing nuclear pore complex architecture with proximity-dependent biotinylation

Proximity-dependent biotin identification (BioID) is a method for identifying protein associations that occur in vivo. By fusing a promiscuous biotin ligase to a protein of interest expressed in living cells, BioID permits the labeling of proximate proteins during a defined labeling period. In this...

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Autors principals: Kim, Dae In, KC, Birendra, Zhu, Wenhong, Motamedchaboki, Khatereh, Doye, Valérie, Roux, Kyle J.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4066523/
https://ncbi.nlm.nih.gov/pubmed/24927568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1406459111
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