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Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation

[Image: see text] Site-specific incorporation of nonstandard amino acids (NSAAs) into proteins enables the creation of biopolymers, proteins, and enzymes with new chemical properties, new structures, and new functions. To achieve this, amber (TAG codon) suppression has been widely applied. However,...

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Main Authors: Hong, Seok Hoon, Ntai, Ioanna, Haimovich, Adrian D., Kelleher, Neil L., Isaacs, Farren J., Jewett, Michael C.
Formato: Artigo
Idioma:Inglês
Publicado: American Chemical Society 2013
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4065633/
https://ncbi.nlm.nih.gov/pubmed/24328168
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/sb400140t
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