Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray

A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligat...

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Autors principals: Moran, Sebastián, Vizoso, Miguel, Martinez-Cardús, Anna, Gomez, Antonio, Matías-Guiu, Xavier, Chiavenna, Sebastián M, Fernandez, Andrés G, Esteller, Manel
Format: Artigo
Idioma:Inglês
Publicat: Landes Bioscience 2014
Matèries:
Brief Report
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4065180/
https://ncbi.nlm.nih.gov/pubmed/24732293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/epi.28790
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