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RISC is a 5′ phosphomonoester-producing RNA endonuclease
Gene silencing in the process of RNA interference is mediated by a ribonucleoprotein complex referred to as RNA-induced silencing complex (RISC). Here we describe the molecular mechanism of target RNA cleavage using affinity-purified minimal RISC from human cells. Cleavage proceeds via hydrolysis an...
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| Main Authors: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Cold Spring Harbor Laboratory Press
2004
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC406288/ https://ncbi.nlm.nih.gov/pubmed/15105377 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.1187904 |
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