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RISC is a 5′ phosphomonoester-producing RNA endonuclease

Gene silencing in the process of RNA interference is mediated by a ribonucleoprotein complex referred to as RNA-induced silencing complex (RISC). Here we describe the molecular mechanism of target RNA cleavage using affinity-purified minimal RISC from human cells. Cleavage proceeds via hydrolysis an...

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Detalhes bibliográficos
Main Authors: Martinez, Javier, Tuschl, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC406288/
https://ncbi.nlm.nih.gov/pubmed/15105377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.1187904
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