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Radioligand Binding to Nanodisc-reconstituted Membrane Transporters by Scintillation Proximity Assay
The scintillation proximity assay (SPA) is a powerful technique for measuring radioligand binding to membrane transporters and has become an integral part of high-throughput drug discovery screening efforts. Here we adapt the method for use with purified LeuT, a prokaryotic secondary transporter, re...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4062192/ https://ncbi.nlm.nih.gov/pubmed/24344975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi401412e |
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