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Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents
INTRODUCTION: Biological samples, pharmaceuticals or food contain proteins, lipids, polymers, ammoniums and macromolecules that alter the detection of infectious agents by DNA amplification techniques (PCR). Moreover the targeted DNA has to be released from the complex cell walls and the compact nuc...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Public Library of Science
2014
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4061000/ https://ncbi.nlm.nih.gov/pubmed/24936792 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0094886 |
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