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Enzymatic Treatment of Specimens before DNA Extraction Directly Influences Molecular Detection of Infectious Agents

INTRODUCTION: Biological samples, pharmaceuticals or food contain proteins, lipids, polymers, ammoniums and macromolecules that alter the detection of infectious agents by DNA amplification techniques (PCR). Moreover the targeted DNA has to be released from the complex cell walls and the compact nuc...

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Detalhes bibliográficos
Main Authors: Goldschmidt, Pablo, Degorge, Sandrine, Merabet, Lilia, Chaumeil, Christine
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4061000/
https://ncbi.nlm.nih.gov/pubmed/24936792
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0094886
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