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FRET Study of the Structural and Kinetic Effects of PKC Phosphomimetic Cardiac Troponin T Mutants on Thin Filament Regulation

FRET was used to investigate the structural and kinetic effects that PKC phosphorylations exert on Ca2+ and myosin subfragment-1 dependent conformational transitions of the cardiac thin filament. PKC phosphorylations of cTnT were mimicked by glutamate substitution. Ca2+ and S1-induced distance chang...

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Detalhes bibliográficos
Main Authors: Schlecht, William, Zhou, Zhiqun, Li, King-Lun, Rieck, Daniel, Ouyang, Yexin, Dong, Wen-Ji
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4059398/
https://ncbi.nlm.nih.gov/pubmed/24708997
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2014.03.013
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