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A New Strategy of RNA Interference That Targets Heterologous Sequences Reveals CITFA1 as an Essential Component of Class I Transcription Factor A in Trypanosoma brucei

Conditional gene silencing by RNA interference in Trypanosoma brucei can be inconclusive if knockdowns are inefficient or have off-target effects. To enable efficient, specific silencing of single-copy genes in mammalian-infective, bloodstream form trypanosomes, we developed a system that targets th...

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Autores principales: Park, Sung Hee, Nguyen, Bao N., Kirkham, Justin K., Nguyen, Tu N., Günzl, Arthur
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2014
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4054280/
https://ncbi.nlm.nih.gov/pubmed/24728195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00014-14
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