RNase-mediated protein footprint sequencing reveals protein-binding sites throughout the human transcriptome

Although numerous approaches have been developed to map RNA-binding sites of individual RNA-binding proteins (RBPs), few methods exist that allow assessment of global RBP–RNA interactions. Here, we describe PIP-seq, a universal, high-throughput, ribonuclease-mediated protein footprint sequencing app...

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Detalhes bibliográficos
Main Authors: Silverman, Ian M, Li, Fan, Alexander, Anissa, Goff, Loyal, Trapnell, Cole, Rinn, John L, Gregory, Brian D
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4053792/
https://ncbi.nlm.nih.gov/pubmed/24393486
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2014-15-1-r3
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