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Efficient CRISPR/Cas9-Mediated Gene Editing in Arabidopsis thaliana and Inheritance of Modified Genes in the T2 and T3 Generations
The newly developed CRISPR/Cas9 system for targeted gene knockout or editing has recently been shown to function in plants in both transient expression systems as well as in primary T1 transgenic plants. However, stable transmission of genes modified by the Cas9/single guide RNA (sgRNA) system to th...
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| Format: | Artigo |
| Sprache: | Inglês |
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Public Library of Science
2014
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4053344/ https://ncbi.nlm.nih.gov/pubmed/24918588 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0099225 |
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