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Double nanohole optical tweezers visualize protein p53 suppressing unzipping of single DNA-hairpins
Here we report on the use of double-nanohole (DNH) optical tweezers as a label-free and free-solution single-molecule probe for protein–DNA interactions. Using this approach, we demonstrate the unzipping of individual 10 base pair DNA-hairpins, and quantify how tumor suppressor p53 protein delays th...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Optical Society of America
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4052917/ https://ncbi.nlm.nih.gov/pubmed/24940547 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.5.001886 |
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