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Observing single protein binding by optical transmission through a double nanohole aperture in a metal film
We experimentally demonstrate protein binding at the single particle level. A double nanohole (DNH) optical trap was used to hold onto a 20 nm biotin-coated polystyrene (PS) particle which subsequently is bound to streptavidin. Biotin-streptavidin binding has been detected by an increase in the opti...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Optical Society of America
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3771822/ https://ncbi.nlm.nih.gov/pubmed/24049672 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.4.001504 |
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