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Impact of scaffold rigidity on the design and evolution of an artificial Diels-Alderase
By combining targeted mutagenesis, computational refinement, and directed evolution, a modestly active, computationally designed Diels-Alderase was converted into the most proficient biocatalyst for [4+2] cycloadditions known. The high stereoselectivity and minimal product inhibition of the evolved...
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| Auteurs principaux: | , , , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
2014
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4050586/ https://ncbi.nlm.nih.gov/pubmed/24847076 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1401073111 |
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