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Impact of scaffold rigidity on the design and evolution of an artificial Diels-Alderase

By combining targeted mutagenesis, computational refinement, and directed evolution, a modestly active, computationally designed Diels-Alderase was converted into the most proficient biocatalyst for [4+2] cycloadditions known. The high stereoselectivity and minimal product inhibition of the evolved...

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Bibliographische Detailangaben
Hauptverfasser: Preiswerk, Nathalie, Beck, Tobias, Schulz, Jessica D., Milovník, Peter, Mayer, Clemens, Siegel, Justin B., Baker, David, Hilvert, Donald
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4050586/
https://ncbi.nlm.nih.gov/pubmed/24847076
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1401073111
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