Analysis of Run-to-Run Variation of Bar-Coded Pyrosequencing for Evaluating Bacterial Community Shifts and Individual Taxa Dynamics

Bar-coded pyrosequencing has been increasingly used due to its fine taxonomic resolution and high throughput. Yet, concerns arise regarding the reproducibility of bar-coded pyrosequencing. We evaluated the run-to-run variation of bar-coded pyrosequencing in detecting bacterial community shifts and t...

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Bibliographic Details
Main Authors: Ge, Yuan, Schimel, Joshua P., Holden, Patricia A.
Format: Artigo
Language:Inglês
Published: Public Library of Science 2014
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4049813/
https://ncbi.nlm.nih.gov/pubmed/24911191
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0099414
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