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Dual primer emulsion PCR for next-generation DNA sequencing

We have developed a highly sensitive single-molecule clonal amplification method called dual primer emulsion PCR (DPePCR) for next-generation DNA sequencing. The approach is similar in concept to standard emulsion PCR; however, in DPePCR both primers are attached to the beads, therefore following PC...

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Bibliografiske detaljer
Main Authors: Xu, Ming Yan, Aragon, Anthony D., Mascarenas, Monica R., Torrez-Martinez, Norah, Edwards, Jeremy S.
Format: Artigo
Sprog:Inglês
Udgivet: 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4049327/
https://ncbi.nlm.nih.gov/pubmed/20569215
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113423
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