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Estimating prion concentration in fluids and tissues by quantitative PMCA

Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samp...

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Detalhes bibliográficos
Main Authors: Chen, Baian, Morales, Rodrigo, Barria, Marcelo A, Soto, Claudio
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4049222/
https://ncbi.nlm.nih.gov/pubmed/20512142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1465
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