Rapid Quantification of Drug Resistance Gene Expression in Candida albicans by Reverse Transcriptase LightCycler PCR and Fluorescent Probe Hybridization

We developed a rapid, sensitive, and reproducible assay to quantify Candida albicans ACT1, CDR1, CDR2, ERG11, and MDR1 mRNA using a two-step reverse transcription and LightCycler real-time PCR (RT-LightCycler PCR) method with sequence-specific hybridization probes. We compared RT-LightCycler PCR wit...

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Bibliographische Detailangaben
Hauptverfasser: Frade, Joao P., Warnock, David W., Arthington-Skaggs, Beth A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2004
Schlagworte:
Mycology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC404661/
https://ncbi.nlm.nih.gov/pubmed/15131174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.5.2085-2093.2004
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