Rapid Quantification of Drug Resistance Gene Expression in Candida albicans by Reverse Transcriptase LightCycler PCR and Fluorescent Probe Hybridization

We developed a rapid, sensitive, and reproducible assay to quantify Candida albicans ACT1, CDR1, CDR2, ERG11, and MDR1 mRNA using a two-step reverse transcription and LightCycler real-time PCR (RT-LightCycler PCR) method with sequence-specific hybridization probes. We compared RT-LightCycler PCR wit...

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Detalles Bibliográficos
Publicado en:J Clin Microbiol
Main Authors: Frade, Joao P., Warnock, David W., Arthington-Skaggs, Beth A.
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 2004
Assuntos:
Mycology
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC404661/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15131174/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.42.5.2085-2093.2004
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