Comparison of a Duplex Quantitative Real-Time PCR Assay and the COBAS Amplicor CMV Monitor Test for Detection of Cytomegalovirus

A duplex quantitative real-time PCR (qPCR) assay was designed to detect both the polymerase gene (pol) and the glycoprotein gene (gB) of cytomegalovirus (CMV). The detection limit of the qPCR was determined to be 1 to 3 copies/reaction and the linear measure interval was 10(3) to 10(8) copies/ml. Th...

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Bibliographic Details
Main Authors: Herrmann, Björn, Larsson, Viviana Cavaglia, Rubin, Carl-Johan, Sund, Fredrik, Eriksson, Britt-Marie, Arvidson, Johan, Yun, Zhibing, Bondeson, Kåre, Blomberg, Jonas
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2004
Subjects:
Virology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC404600/
https://ncbi.nlm.nih.gov/pubmed/15131148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.5.1909-1914.2004
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