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Comparison of a Duplex Quantitative Real-Time PCR Assay and the COBAS Amplicor CMV Monitor Test for Detection of Cytomegalovirus

A duplex quantitative real-time PCR (qPCR) assay was designed to detect both the polymerase gene (pol) and the glycoprotein gene (gB) of cytomegalovirus (CMV). The detection limit of the qPCR was determined to be 1 to 3 copies/reaction and the linear measure interval was 10(3) to 10(8) copies/ml. Th...

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Dettagli Bibliografici
Pubblicato in:J Clin Microbiol
Autori principali: Herrmann, Björn, Larsson, Viviana Cavaglia, Rubin, Carl-Johan, Sund, Fredrik, Eriksson, Britt-Marie, Arvidson, Johan, Yun, Zhibing, Bondeson, Kåre, Blomberg, Jonas
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology (ASM) 2004
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC404600/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15131148/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.42.5.1909-1914.2004
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