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Selective and flexible depletion of problematic sequences from RNA-seq libraries at the cDNA stage
BACKGROUND: A major hurdle to transcriptome profiling by deep-sequencing technologies is that abundant transcripts, such as rRNAs, can overwhelm the libraries, severely reducing transcriptome-wide coverage. Methods for depletion of such unwanted sequences typically require treatment of RNA samples p...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4045971/ https://ncbi.nlm.nih.gov/pubmed/24886553 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-15-401 |
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