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Mechanistic Study of Manganese-Substituted Glycerol Dehydrogenase Using a Kinetic and Thermodynamic Analysis

Mechanistic insights regarding the activity enhancement of dehydrogenase by metal ion substitution were investigated by a simple method using a kinetic and thermodynamic analysis. By profiling the binding energy of both the substrate and product, the metal ion's role in catalysis enhancement wa...

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Autors principals: Fang, Baishan, Niu, Jin, Ren, Hong, Guo, Yingxia, Wang, Shizhen
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4045801/
https://ncbi.nlm.nih.gov/pubmed/24896258
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0099162
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