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Sucrose Increases the Activation Energy Barrier for Actin-Myosin Strong Binding
To determine the mechanism by which sucrose slows in vitro actin sliding velocities, V, we used stopped flow kinetics and a single molecule binding assay, SiMBA. We observed that in the absence of ATP, sucrose (880 mM) slowed the rate of actin-myosin (A-M) strong binding by 71 ± 8% with a smaller in...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4043939/ https://ncbi.nlm.nih.gov/pubmed/24370736 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2013.12.012 |
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