Sucrose Increases the Activation Energy Barrier for Actin-Myosin Strong Binding

To determine the mechanism by which sucrose slows in vitro actin sliding velocities, V, we used stopped flow kinetics and a single molecule binding assay, SiMBA. We observed that in the absence of ATP, sucrose (880 mM) slowed the rate of actin-myosin (A-M) strong binding by 71 ± 8% with a smaller in...

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Detaylı Bibliyografya
Asıl Yazarlar: Jackson, Del R., Webb, Milad, Stewart, Travis J., Phillips, Travis, Carter, Michael, Cremo, Christine R., Baker, Josh E.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2013
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Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4043939/
https://ncbi.nlm.nih.gov/pubmed/24370736
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2013.12.012
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