Flow Cytometry Enables A High-Throughput Homogeneous Fluorescent Antibody-Binding Assay for Cytotoxic T Cell Lytic Granule Exocytosis

We developed a homogeneous phenotypic fluorescence endpoint assay for cytotoxic T lymphocyte lytic granule exocytosis. This flow cytometric assay measures binding of an antibody to a luminal epitope of a lysosomal membrane protein (LAMP-1) that is exposed by exocytosis to the extracellular solution....

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Bibliographic Details
Main Authors: Florian, Amy E., Lepensky, Christopher K., Kwon, Ohyun, Haynes, Mark K., Sklar, Larry A., Zweifach, Adam
Format: Artigo
Language:Inglês
Published: 2012
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4043149/
https://ncbi.nlm.nih.gov/pubmed/23160568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1087057112466697
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