Inhibition Controls for Qualitative Real-Time PCR Assays: Are They Necessary for All Specimen Matrices?

A retrospective analysis of 386,706 specimens representing a variety of matrix types used in qualitative real-time PCR assays determined the overall inhibition rate to be 0.87% when the inhibition control was added preextraction to 5,613 specimens and 0.01% when the inhibition control was added post...

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Detalles Bibliográficos
Autores principales: Buckwalter, S. P., Sloan, L. M., Cunningham, S. A., Espy, M. J., Uhl, J. R., Jones, M. F., Vetter, E. A., Mandrekar, J., Cockerill, F. R., Pritt, B. S., Patel, R., Wengenack, N. L.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2014
Materias:
Bacteriology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4042775/
https://ncbi.nlm.nih.gov/pubmed/24740078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.03389-13
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