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Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay to the Presence of Urea

A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤1.8 M, compared with a PCR assay that was functional at concentrations of <100 mM. The LAMP assay was as sen...

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Detalhes bibliográficos
Main Authors: Edwards, Thomas, Burke, Patricia A., Smalley, Helen B., Gillies, Liz, Hobbs, Glyn
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4042760/
https://ncbi.nlm.nih.gov/pubmed/24622100
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00314-14
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