A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine

We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher tha...

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Detalhes bibliográficos
Main Authors: Koizumi, Nobuo, Nakajima, Chie, Harunari, Tsunehito, Tanikawa, Tsutomu, Tokiwa, Toshihiro, Uchimura, Eriko, Furuya, Tokujiro, Mingala, Claro Niegos, Villanueva, Marvin Ardeza, Ohnishi, Makoto, Suzuki, Yasuhiko
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3372145/
https://ncbi.nlm.nih.gov/pubmed/22422858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00481-12
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