A New Loop-Mediated Isothermal Amplification Method for Rapid, Simple, and Sensitive Detection of Leptospira spp. in Urine

We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher tha...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Koizumi, Nobuo, Nakajima, Chie, Harunari, Tsunehito, Tanikawa, Tsutomu, Tokiwa, Toshihiro, Uchimura, Eriko, Furuya, Tokujiro, Mingala, Claro Niegos, Villanueva, Marvin Ardeza, Ohnishi, Makoto, Suzuki, Yasuhiko
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 2012
Pynciau:
Bacteriology
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3372145/
https://ncbi.nlm.nih.gov/pubmed/22422858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00481-12
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