Label-free Relative Quantification of Alpha-2-macroglobulin site-specific Core-fucosylation in Pancreatic Cancer by LC-MS/MS

We describe a label-free relative quantification LC-MS/MS method for core-fucosylationin alpha-2-macroglobulin (A2MG) immunoprecipitated from human sera. The method utilizes endoglycosidase F partial deglycosylation to reduce glycosylation microheterogeneity, while retaining the innermost N-acetylgl...

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Detalhes bibliográficos
Main Authors: Lin, Zhenxin, Yin, Haidi, Lo, Andy, Ruffin, Mack T., Anderson, Michelle A., Simeone, Diane M., Lubman, David M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4035462/
https://ncbi.nlm.nih.gov/pubmed/24285556
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.201300376
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