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A method for distance determination in proteins using a designed metal ion binding site and site-directed spin labeling: evaluation with T4 lysozyme.

The use of molecular genetics to introduce both a metal ion binding site and a nitroxide spin label into the same protein opens the use of paramagnetic metalnitroxyl interactions to estimate intramolecular distances in a wide variety of proteins. In this report, a His-Xaa3-His metal ion binding moti...

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Detalhes bibliográficos
Main Authors: Voss, J, Salwiński, L, Kaback, H R, Hubbell, W L
Formato: Artigo
Idioma:Inglês
Publicado em: 1995
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC40343/
https://ncbi.nlm.nih.gov/pubmed/8618888
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