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Internally labeled Cy3/Cy5 DNA constructs show greatly enhanced photo-stability in single-molecule FRET experiments

DNA constructs labeled with cyanine fluorescent dyes are important substrates for single-molecule (sm) studies of the functional activity of protein–DNA complexes. We previously studied the local DNA backbone fluctuations of replication fork and primer–template DNA constructs labeled with Cy3/Cy5 do...

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Detalhes bibliográficos
Main Authors: Lee, Wonbae, von Hippel, Peter H., Marcus, Andrew H.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4027219/
https://ncbi.nlm.nih.gov/pubmed/24627223
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku199
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