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Complete steady-state rate equation for DNA ligase and its use for measuring product kinetic parameters of NAD(+)-dependent DNA ligase from Haemophilus influenzae
BACKGROUND: DNA ligase seals the nicks in the phosphodiester backbone between Okazaki fragments during DNA replication. DNA ligase has an unusual Bi Ter Ping Pong kinetic mechanism. Its substrates in eubacteria are NAD(+) and nicked DNA (nDNA). Its products are nicotinamide mononucleotide (NMN), ade...
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
BioMed Central
2014
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4022432/ https://ncbi.nlm.nih.gov/pubmed/24885075 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-7-287 |
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