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Analysis of optimized DNase-seq reveals intrinsic bias in transcription factor footprint identification
DNase-seq is a powerful technique for identifying cis-regulatory elements across the genome. We studied the key experimental parameters to optimize the performance of DNase-seq. We found that sequencing short 50-100bp fragments that accumulate in long inter-nucleosome linker regions is more efficien...
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| Main Authors: | , , , , , , , , , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2013
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4018771/ https://ncbi.nlm.nih.gov/pubmed/24317252 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2762 |
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