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Analysis of optimized DNase-seq reveals intrinsic bias in transcription factor footprint identification

DNase-seq is a powerful technique for identifying cis-regulatory elements across the genome. We studied the key experimental parameters to optimize the performance of DNase-seq. We found that sequencing short 50-100bp fragments that accumulate in long inter-nucleosome linker regions is more efficien...

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Detalhes bibliográficos
Main Authors: He, Housheng Hansen, Meyer, Clifford A., Hu, Sheng'en Shawn, Chen, Mei-Wei, Zang, Chongzhi, Liu, Yin, Rao, Prakash K., Fei, Teng, Xu, Han, Long, Henry, Liu, X. Shirley, Brown, Myles
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4018771/
https://ncbi.nlm.nih.gov/pubmed/24317252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2762
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