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Quantitative Measurement of Brightness from Living Cells in the Presence of Photodepletion

The brightness of fluorescently labeled proteins provides an excellent marker for identifying protein interactions in living cells. Quantitative interpretation of brightness, however, hinges on a detailed understanding of the processes that affect the signal fluctuation of the fluorescent label. Her...

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Detalles Bibliográficos
Main Authors: Hur, Kwang-Ho, Macdonald, Patrick J., Berk, Serkan, Angert, C. Isaac, Chen, Yan, Mueller, Joachim D.
Formato: Artigo
Idioma:Inglês
Publicado: Public Library of Science 2014
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4018325/
https://ncbi.nlm.nih.gov/pubmed/24820174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0097440
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