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Quantitative Measurement of Brightness from Living Cells in the Presence of Photodepletion

The brightness of fluorescently labeled proteins provides an excellent marker for identifying protein interactions in living cells. Quantitative interpretation of brightness, however, hinges on a detailed understanding of the processes that affect the signal fluctuation of the fluorescent label. Her...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Hur, Kwang-Ho, Macdonald, Patrick J., Berk, Serkan, Angert, C. Isaac, Chen, Yan, Mueller, Joachim D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4018325/
https://ncbi.nlm.nih.gov/pubmed/24820174
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0097440
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