Mapping U2 snRNP--pre-mRNA interactions using biotinylated oligonucleotides made of 2'-OMe RNA.

Biotinylated 2'-OMe RNA oligonucleotides complementary to two separate regions of human U2 snRNA have been used as affinity probes to study U2 snRNP--pre-mRNA interactions. Both oligonucleotides bind specifically and allow highly selective removal of U2 snRNP from HeLa cell nuclear extracts. Pre-mRN...

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Detalles Bibliográficos

Publicado en:	EMBO J
Autores principales:	Barabino, S M, Sproat, B S, Ryder, U, Blencowe, B J, Lamond, A I
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Nature Publishing Group 1989
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC401608/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2531659/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1989.tb08602.x
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