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Mapping U2 snRNP--pre-mRNA interactions using biotinylated oligonucleotides made of 2'-OMe RNA.

Biotinylated 2'-OMe RNA oligonucleotides complementary to two separate regions of human U2 snRNA have been used as affinity probes to study U2 snRNP--pre-mRNA interactions. Both oligonucleotides bind specifically and allow highly selective removal of U2 snRNP from HeLa cell nuclear extracts. Pr...

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Dades bibliogràfiques
Publicat a:EMBO J
Autors principals: Barabino, S M, Sproat, B S, Ryder, U, Blencowe, B J, Lamond, A I
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 1989
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC401608/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2531659/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1989.tb08602.x
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