Exploring Codon Optimization and Response Surface Methodology to Express Biologically Active Transmembrane RANKL in E. coli

Receptor activator of nuclear factor (NF)-κB ligand (RANKL), a master cytokine that drives osteoclast differentiation, activation and survival, exists in both transmembrane and extracellular forms. To date, studies on physiological role of RANKL have been mainly carried out with extracellular RANKL...

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Bibliografske podrobnosti
Main Authors: Maharjan, Sushila, Singh, Bijay, Bok, Jin-Duck, Kim, Jeong-In, Jiang, Tao, Cho, Chong-Su, Kang, Sang-Kee, Choi, Yun-Jaie
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2014
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4014495/
https://ncbi.nlm.nih.gov/pubmed/24809485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0096259
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