Exploring Codon Optimization and Response Surface Methodology to Express Biologically Active Transmembrane RANKL in E. coli

Receptor activator of nuclear factor (NF)-κB ligand (RANKL), a master cytokine that drives osteoclast differentiation, activation and survival, exists in both transmembrane and extracellular forms. To date, studies on physiological role of RANKL have been mainly carried out with extracellular RANKL...

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Detalhes bibliográficos
Main Authors: Maharjan, Sushila, Singh, Bijay, Bok, Jin-Duck, Kim, Jeong-In, Jiang, Tao, Cho, Chong-Su, Kang, Sang-Kee, Choi, Yun-Jaie
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4014495/
https://ncbi.nlm.nih.gov/pubmed/24809485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0096259
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