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Expression and Purification of the scFv from Hybridoma Cells Secreting a Monoclonal Antibody Against S Protein of PEDV

The variable regions of the heavy chain (VH) and light chain (VL) were amplified by RT-PCR from the hybridoma 6E6, which secretes the monoclonal antibody against PEDV S protein. The VL and VH amplicons were combined using SOE-PCR by a 12 amino acid flexible linker (SSGGGGSGGGGS), which produced the...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Zhu, Qinghe, Guo, Donghua, Feng, Li, Sun, Dongbo
פורמט: Artigo
שפה:Inglês
יצא לאור: Mary Ann Liebert, Inc. 2013
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC4014299/
https://ncbi.nlm.nih.gov/pubmed/23600505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/mab.2012.0089
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