Quantitative, Time-Resolved Proteomic Analysis by Combining Bioorthogonal Noncanonical Amino Acid Tagging and Pulsed Stable Isotope Labeling by Amino Acids in Cell Culture

An approach to proteomic analysis that combines bioorthogonal noncanonical amino acid tagging (BONCAT) and pulsed stable isotope labeling with amino acids in cell culture (pSILAC) provides accurate quantitative information about rates of cellular protein synthesis on time scales of minutes. The meth...

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Bibliografiske detaljer
Main Authors: Bagert, John D., Xie, Yushu J., Sweredoski, Michael J., Qi, Yutao, Hess, Sonja, Schuman, Erin M., Tirrell, David A.
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Biochemistry and Molecular Biology 2014
Fag:
Technological Innovation and Resources
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4014290/
https://ncbi.nlm.nih.gov/pubmed/24563536
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M113.031914
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