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Quantitative, Time-Resolved Proteomic Analysis by Combining Bioorthogonal Noncanonical Amino Acid Tagging and Pulsed Stable Isotope Labeling by Amino Acids in Cell Culture

An approach to proteomic analysis that combines bioorthogonal noncanonical amino acid tagging (BONCAT) and pulsed stable isotope labeling with amino acids in cell culture (pSILAC) provides accurate quantitative information about rates of cellular protein synthesis on time scales of minutes. The meth...

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Autori principali: Bagert, John D., Xie, Yushu J., Sweredoski, Michael J., Qi, Yutao, Hess, Sonja, Schuman, Erin M., Tirrell, David A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Biochemistry and Molecular Biology 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4014290/
https://ncbi.nlm.nih.gov/pubmed/24563536
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M113.031914
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