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Evidence from complementation assays in vitro that U5 snRNP is required for both steps of mRNA splicing.

We have established an in vitro complementation system that has allowed us to investigate the role of individual purified snRNPs in the splicing of pre-mRNA molecules. For the preparation of snRNP-depleted nuclear extracts we have first removed the majority of endogenous snRNPs from the nuclear extr...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:EMBO J
Prif Awduron: Winkelmann, G, Bach, M, Lührmann, R
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Nature Publishing Group 1989
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC401390/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2531074/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1989.tb08462.x
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