Evidence from complementation assays in vitro that U5 snRNP is required for both steps of mRNA splicing.
We have established an in vitro complementation system that has allowed us to investigate the role of individual purified snRNPs in the splicing of pre-mRNA molecules. For the preparation of snRNP-depleted nuclear extracts we have first removed the majority of endogenous snRNPs from the nuclear extr...
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| Vydáno v: | EMBO J |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Nature Publishing Group
1989
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC401390/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2531074/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1989.tb08462.x |
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