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Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR
A frequency-modulated fluorescence encoding method was used as a means to increase the number of fluorophores monitored during infrared-mediated polymerase chain reaction. Laser lines at 488-nm and 561-nm were modulated at 73- and 137-Hz, respectively, exciting fluorescence from the dsDNA intercalat...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4013171/ https://ncbi.nlm.nih.gov/pubmed/24448431 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c3an02334f |
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